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pcr amplification instrument  (Eppendorf AG)


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    Eppendorf AG pcr amplification instrument
    Pcr Amplification Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+instrument/Eppendorf/pm42118147-289-6-9
    Average 99 stars, based on 200557 article reviews
    pcr amplification instrument - by Bioz Stars, 2026-09
    99/100 stars

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    Reverse Transcription:

    Article Title: Specific redox homeostasis, ion accumulation and gas exchange responses in tomato plants subjected to combined drought and salinity stresses
    Article Snippet: The reaction system was configured using HiScript II 1st strand cDNA Synthesis Kit (Vazyme, Nanjing, China). .. Reverse transcription was accomplished using PCR instrument (Mastercycler pro, Eppendorf, Germany), and the cDNA products were stored at − 20 ◦C. ..

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Polymerase Chain Reaction:

    Article Title: Specific redox homeostasis, ion accumulation and gas exchange responses in tomato plants subjected to combined drought and salinity stresses
    Article Snippet: The reaction system was configured using HiScript II 1st strand cDNA Synthesis Kit (Vazyme, Nanjing, China). .. Reverse transcription was accomplished using PCR instrument (Mastercycler pro, Eppendorf, Germany), and the cDNA products were stored at − 20 ◦C. ..

    Article Title: Protocol for KAT8 conditional knockout mice generation and its application in 4NQO-induced esophageal tumor model
    Article Snippet: .. PCR instrument , Eppendorf , Mastercycler nexus. .. Microscope , Olympus , BX43.

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer.
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Article Title: Catechin hydrate prevents cadmium-induced collagen downregulation in mesenchymal stem cells via SMAD/RUNX2
    Article Snippet: Following total RNA extraction using an extraction kit (Super RNA extraction kit, YT9080, Yeasen Biotechnology, China), the reverse transcription PCR (RT-PCR) was carried out to synthesize cDNA with the help of BioFACT kit (BR631-096, BioFACT Co., Ltd, South Korea). .. Amplification of ALP (Alp) , osteocalcin (Oc) , runt-related transcription factor 2 (Runx2), Smadl , bone morphogenetic protein 2 (Bmp2) , collagen-1A1 (CoUaV), and glyceraldehyde dehydrogenase (Gapdh) were performed using a PCR instrument (Eppendorf Mastercycler gradient, Eppendorf Co., Hamburg, Germany) in triplicate by their specific primers ( ). .. The PCR was carried out at 95 °C for 5 min as the initial stage, followed by 35 cycles consisting of 95 °C for 1 min, annealing temperature of specific primer for 1 min, and 72 °C for 1 min. At the final stage, the elongation temperature was set as 72 °C for 7 min. PCR product was checked using a 1.5% agarose gel, then the results were visualized and photographed by gel documentation (Gel Flash, SYNGENE Bio Imaging, England) and further analyzed by Gel Quant software (version 1.8.2) ( ).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: DOTAP-functionalized DNA nanocages enable efficient miR168d trafficking to antagonize PVY infection by modulating HSP90-5 homeostasis
    Article Snippet: .. The mixed liquid was heated to 95 °C for 10 min in a PCR instrument (Eppendorf, Hamburg, Germany) and then cooled to 4 °C for 30 min to form a TDN with the miR168d linker sequence. ..

    Nuclear Magnetic Resonance:

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer.
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Mass Spectrometry:

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer.
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    High Performance Liquid Chromatography:

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer.
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Article Title: Actinolactomycin from Streptomyces sp. CB00271: Isolation, Identification, and Potent Anti-Triple-Negative Breast Cancer.
    Article Snippet: The CCK-8 cell proliferation assay kit was from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). .. Instrumentation included: NMR spectrometers (400/500 MHz, Bruker Corporation, Billerica, MA, USA); a high-resolution mass spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA); HPLC, semi-preparative HPLC, and LC-MS systems (Waters Technologies (Shanghai) Ltd., Shanghai, China); a rotary evaporator (Hangzhou Yijie Technology Co., Ltd., Hangzhou, China); a biochemical incubator (Shanghai Boxun Industry & Commerce Co., Ltd. Medical Equipment Factory, Shanghai, China); a shaking incubator (Shanghai Zhicheng Analytics Instrument Manufacture Co., Ltd., Shanghai, China); and a PCR instrument (Eppendorf AG, Hamburg, Germany). ..

    Control:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Multiple Displacement Amplification:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Protein Concentration:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Software:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Fluorescence:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Real-time Polymerase Chain Reaction:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    RNA Extraction:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Mann-Whitney U-Test:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Amplification:

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Article Title: Catechin hydrate prevents cadmium-induced collagen downregulation in mesenchymal stem cells via SMAD/RUNX2
    Article Snippet: Following total RNA extraction using an extraction kit (Super RNA extraction kit, YT9080, Yeasen Biotechnology, China), the reverse transcription PCR (RT-PCR) was carried out to synthesize cDNA with the help of BioFACT kit (BR631-096, BioFACT Co., Ltd, South Korea). .. Amplification of ALP (Alp) , osteocalcin (Oc) , runt-related transcription factor 2 (Runx2), Smadl , bone morphogenetic protein 2 (Bmp2) , collagen-1A1 (CoUaV), and glyceraldehyde dehydrogenase (Gapdh) were performed using a PCR instrument (Eppendorf Mastercycler gradient, Eppendorf Co., Hamburg, Germany) in triplicate by their specific primers ( ). .. The PCR was carried out at 95 °C for 5 min as the initial stage, followed by 35 cycles consisting of 95 °C for 1 min, annealing temperature of specific primer for 1 min, and 72 °C for 1 min. At the final stage, the elongation temperature was set as 72 °C for 7 min. PCR product was checked using a 1.5% agarose gel, then the results were visualized and photographed by gel documentation (Gel Flash, SYNGENE Bio Imaging, England) and further analyzed by Gel Quant software (version 1.8.2) ( ).

    Article Title: Melatonin alleviates chronic intermittent hypoxia-induced gastric mucosal injury via attenuation of oxidative stress and JNK-mediated apoptotic signaling in rats.
    Article Snippet: .. 48 clean-grade healthy adult male Wistar rats, weighing 190 ± 10g, purchased from Beijing Viton Lihua Laboratory Animal Science and Technology Company Limited (License No.: SCXK (Beijing) 2016−0002), hypoxia chamber (Beijing Xitianlong Co., Ltd.), program control and monitoring system (Tangshan Youyi Science and Technology Co., Ltd.), oil-free lubricated air compressor (Shanghai People’s Group Industry Co., Ltd.), oxygen meter SMART (Xiamen Xinrui Ltd), Oxygen Meter SMART (Xiamen Xinrui Instrumentation Co.),MT was purchased from Sigma-aldrich, slicer (Leica, Germany), blood gas analyzer (Central Laboratory of North China University of Science and Technology), superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, and BCA protein concentration determination kit (AndyGene Biotechnology Co., Ltd.), Reverse transcription instrument (BIO RAD, USA), PCR instrument (Eppendorf, Germany), Rotor-Gene3000 analysis software (Eppendorf, Germany), real-time fluorescence quantitative polymerase chain reaction instrument (Leica, Germany), RNA primer (Beijing Ruibo Xingke Biotechnology Co., Ltd.), RNA reverse transcription kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.), total RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.). .. RNA extraction kit (Wuhan U-Test Biotechnology Co., Ltd.), PCR amplification kit (Beijing Zhuangmeng International Biogenetic Technology Co., Ltd.).

    Sequencing:

    Article Title: DOTAP-functionalized DNA nanocages enable efficient miR168d trafficking to antagonize PVY infection by modulating HSP90-5 homeostasis
    Article Snippet: .. The mixed liquid was heated to 95 °C for 10 min in a PCR instrument (Eppendorf, Hamburg, Germany) and then cooled to 4 °C for 30 min to form a TDN with the miR168d linker sequence. ..



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    Journal: Biology

    Article Title: One-Pot LAMP-Coupled CRISPR/Cas12b Assay Enables Sensitive Detection of Helicobacter pylori

    doi: 10.3390/biology15100797

    Figure Lengend Snippet: LAMP Primer Panel Screening. ( a ) Amplification curves for real-time LAMP; ( b ) agarose gel electrophoresis. CagA2/4/7: Positive controls for the 2nd, 4th, and 7th sets of CagA LAMP primers, respectively; NTC-CagA2/4/7: Negative controls for the 2nd, 4th, and 7th sets of CagA LAMP primers, respectively.

    Article Snippet: The key instruments applied in this investigation included a −80 °C ultra-low temperature freezer (Aucma, Qingdao, China), a high-speed microcentrifuge (Michael Laboratory Instrument, Changsha, China), a microspectrophotometer (Xinling Biotechnology, Shanghai, China), micropipettes (Eppendorf, Hamburg, Germany), a biosafety cabinet (Xinbeixi Biotechnology, Jinan, China), Real-time fluorescence quantitative PCR(qPCR) instrument (Bioer Technology, Hangzhou, China), an electronic balance (Puchun Instrument, Shanghai, China), an electrophoresis apparatus (Yixin Analytical Instrument, Shanghai, China), and a gel imaging system (Yixin Analytical Instrument, Shanghai, China).

    Techniques: Amplification, Agarose Gel Electrophoresis